Figure 2

CD34+ cell purity and transduction efficiency: CD34+ cells derived from human fetal liver were purified by immunomagnetic beads and assayed by FACS. Percentage purity is indicated in A. Purified CD34+ cells were transduced with HIV-1 Tar (B) and Tar-CCR5 ribozyme (C) containing lentiviral vector. Percentages of EGFP positive cells at 48 hrs post-transduction are indicated in panel B and C. Isotype antibody control is shown in each panel (unshaded areas).