Figure 5

HIV-1 challenge of differentiated macrophages: HIV-1 Tar, Tar-CCR5Rz and control EGFP vector transduced and unmanipulated control CD34+ cells were allowed to differentiate into macrophages in vitro. Later, they were challenged with a macrophage tropic HIV-1 strain BaL. Viral supernatants were collected at different times post-infection and assayed for p24 antigen by ELISA. Values represent averages of duplicate cultures.