Figure 2

Stably transduced CEM clones expressing the U16RBEdecoy. The CEM cells were first transduced with the pBabe constructs and then selected for Puromycin resistance.

From the resulting pooled population single clones were selected by limiting dilution. Total RNA isolated from the single clones was electrophoresed either in a 6% polyacrylamide-7M Urea (A) or 1.2 % Agarose formaldehyde (B) gels. The electrophoresed RNAs were blotted onto a nylon filter followed by hybridization with specific probes (see Material and Methods).