Figure 4

Scatter plots showing CD3+ T cell gating and CD3+CD4+ T cells in EasyCD4 System. Plot A: CD3+ cells (in red) are gated using the size (FSC: X axis) and the CD3-PECy5 staining (PM2: Y axis) using the threshold setting markers. Plot B: CD3+CD4+ T cells are gated in CD4 analysis gate using two-color fluorescence CD4- PE (PM1: X axis) and CD3- PECy5 (PM2: Y axis) using the threshold setting markers.