HIV drug resistance: analysis of viral genotypes and mutation loci in people living with HIV in Chongqing, China (2016–2023)

Table 1 Gene amplification and sequencing primers for HIV-1 pol

Procedure	Primers	Position^a	Length of target fragment	Sequences (5’-3’)
First round	2029 F	2029–2050	1501	TGGAAATGTGGRAAGGAAGGAC
	3529R	3529–3505		GCTAyyAAGTCTTTTGATGGGTCAT
Second round	2249 F	2249–2266	1273	CTTCCCTCARATCACTCT
	3521R	3521–3504		GTCTTTTGATGGGTCATA

Positions ^a: Positions of nucleotides within the HIV HXB2 strain (GenBank accession number: K03455)

ISSN: 1742-6405